6J6J

Biotin-bound streptavidin

Summary for 6J6J

• Entry DOI: 10.2210/pdb6j6j/pdb
• EMDB information: 0689 0690
• Descriptor: Streptavidin, BIOTIN (3 entities in total)
• Functional Keywords: streptavidin, cytosolic protein
• Biological source: Streptomyces avidinii
• Total number of polymer chains: 4
• Total formula weight: 51363.81

Authors

Fan, X.,Wang, J.,Lei, J.L.,Wang, H.W. (deposition date: 2019-01-15, release date: 2019-05-29, Last modification date: 2024-03-27)

Primary citation

Fan, X.,Wang, J.,Zhang, X.,Yang, Z.,Zhang, J.C.,Zhao, L.,Peng, H.L.,Lei, J.,Wang, H.W.
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.
Nat Commun, 10:2386-2386, 2019

PubMed Abstract: The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.

PubMed: 31160591
DOI: 10.1038/s41467-019-10368-w

Experimental method

ELECTRON MICROSCOPY (3.2 Å)