6J2S

Structure of HitA bound to gallium from Pseudomonas aeruginosa

Summary for 6J2S

• Entry DOI: 10.2210/pdb6j2s/pdb
• Descriptor: Ferric iron-binding periplasmic protein, PHOSPHATE ION, GALLIUM (III) ION, ... (4 entities in total)
• Functional Keywords: hita, gallium, metal-drug, ferric iron binding protein, transporter, metal binding protein
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 2
• Total formula weight: 66746.46

Authors

Guo, Y.,Li, H.Y.,Sun, H.Z.,Xia, W. (deposition date: 2019-01-02, release date: 2020-01-01, Last modification date: 2023-11-22)

Primary citation

Guo, Y.,Li, W.,Li, H.,Xia, W.
Identification and Characterization of a Metalloprotein Involved in Gallium Internalization in Pseudomonas aeruginosa.
Acs Infect Dis., 5:1693-1697, 2019

PubMed Abstract: Gallium nitrate (Ganite) is a potential drug for the treatment of infection. CRISPR/Cas9-based gene mutagenesis studies reveal that siderophore pyochelin-facilitated uptake and an ABC transporter are two major Ga internalization pathways in (). Crystal structures reveal that Ga and Fe occupy exactly the same metal site of HitA, a periplasmic iron-binding protein of the ABC transporter system. The study provides a molecular basis for Ga internalization by and facilitates gallium-based antimicrobial drug development.

PubMed: 31475514
DOI: 10.1021/acsinfecdis.9b00271

Experimental method

X-RAY DIFFRACTION (1.73005117666 Å)