6ITP

Crystal structure of cortisol complexed with its nanobody at pH 3.5

6ITP の概要

• エントリーDOI: 10.2210/pdb6itp/pdb
• 分子名称: anti-cortisol camelid antibody, (11alpha,14beta)-11,17,21-trihydroxypregn-4-ene-3,20-dione (3 entities in total)
• 機能のキーワード: cortisol, complex, nanobody, camelid antibody, immune system
• 由来する生物種: Camelus bactrianus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28285.11

構造登録者

Ding, Y.,Ding, L.L.,Wang, Z.Y.,Zhong, P.Y. (登録日: 2018-11-24, 公開日: 2019-07-24, 最終更新日: 2023-11-22)

主引用文献

Ding, L.,Wang, Z.,Zhong, P.,Jiang, H.,Zhao, Z.,Zhang, Y.,Ren, Z.,Ding, Y.
Structural insights into the mechanism of single domain VHH antibody binding to cortisol.
Febs Lett., 593:1248-1256, 2019

PubMed Abstract: To date, few structural models of VHH antibody binding to low molecular weight haptens have been reported. Here, we report the crystal structure of cortisol binding to its VHH antibody NbCor at pH 3.5 and 10.5. Cortisol binds to NbCor mainly by burying itself under the tunnel formed by the complementarity determining region 1 (CDR1) of NbCor. The affinity of NbCor binding to cortisol and similar compounds was also verified by a microscale thermophoresis assay. Combining our findings with several previously reported structures of hapten-VHH antibody complexes, we propose that VHH antibodies exhibit a special mechanism of binding small haptens by encapsulating them in a tunnel formed by CDR1. Our findings provide useful structural information for the further development and optimization of hapten-specific VHH antibodies.

PubMed: 31049949
DOI: 10.1002/1873-3468.13398

実験手法

X-RAY DIFFRACTION (1.572 Å)