6ISO

Human SIRT3 Recognizing H3K4cr

「4V1C」から置き換えられました

6ISO の概要

• エントリーDOI: 10.2210/pdb6iso/pdb
• 分子名称: NAD-dependent protein deacetylase sirtuin-3, mitochondrial, ARG-THR-LYS-GLN-THR-ALA-ARG, ZINC ION, ... (6 entities in total)
• 機能のキーワード: posttranslational modification, sirtuins, histones, complex., hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 189691.77

構造登録者

Wang, Y.,Hao, Q. (登録日: 2018-11-17, 公開日: 2019-01-23, 最終更新日: 2024-11-20)

主引用文献

Bao, X.,Wang, Y.,Li, X.,Li, X.M.,Liu, Z.,Yang, T.,Wong, C.F.,Zhang, J.,Hao, Q.,Li, X.D.
Identification of 'erasers' for lysine crotonylated histone marks using a chemical proteomics approach.
Elife, 3:-, 2014

PubMed Abstract: Posttranslational modifications (PTMs) play a crucial role in a wide range of biological processes. Lysine crotonylation (Kcr) is a newly discovered histone PTM that is enriched at active gene promoters and potential enhancers in mammalian cell genomes. However, the cellular enzymes that regulate the addition and removal of Kcr are unknown, which has hindered further investigation of its cellular functions. Here we used a chemical proteomics approach to comprehensively profile 'eraser' enzymes that recognize a lysine-4 crotonylated histone H3 (H3K4Cr) mark. We found that Sirt1, Sirt2, and Sirt3 can catalyze the hydrolysis of lysine crotonylated histone peptides and proteins. More importantly, Sirt3 functions as a decrotonylase to regulate histone Kcr dynamics and gene transcription in living cells. This discovery not only opens opportunities for examining the physiological significance of histone Kcr, but also helps to unravel the unknown cellular mechanisms controlled by Sirt3, that have previously been considered solely as a deacetylase.

PubMed: 25369635
DOI: 10.7554/eLife.02999

実験手法

X-RAY DIFFRACTION (2.95 Å)