6IQO

Se-Met L45M Programmed Cell Death 5 protein from Sulfolobus solfataricus

6IQO の概要

• エントリーDOI: 10.2210/pdb6iqo/pdb
• 分子名称: DNA-binding protein SSO0352, NONAETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: dna binding protein, sulfolobus, programmed cell death
• 由来する生物種: Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30452.00

構造登録者

Chen, C.Y.,Lin, K.F.,Hsu, C.Y.,Tsai, M.J. (登録日: 2018-11-08, 公開日: 2019-02-20, 最終更新日: 2024-10-30)

主引用文献

Lin, K.F.,Hsu, J.Y.,Hsieh, D.L.,Tsai, M.J.,Yeh, C.H.,Chen, C.Y.
Crystal structure of the programmed cell death 5 protein from Sulfolobus solfataricus.
Acta Crystallogr F Struct Biol Commun, 75:73-79, 2019

PubMed Abstract: Programmed cell death 5 (PDCD5) is a vital signaling protein in the apoptosis pathway in eukaryotes. It is known that there are two dissociated N-terminal regions and a triple-helix core in eukaryotic PDCD5. Structural and functional studies of PDCD5 from hyperthermophilic archaea have been limited to date. Here, the PDCD5 homolog Sso0352 (SsoPDCD5) was identified in Sulfolobus solfataricus, the SsoPDCD5 protein was expressed and crystallized, and the phase was identified by single-wavelength anomalous diffraction. The native SsoPDCD5 crystal belonged to space group C2 and diffracted to 1.49 Å resolution. This is the first crystal structure of a PDCD5 homolog to be solved. SsoPDCD5 shares a similar triple-helix bundle with eukaryotic PDCD5 but has a long α-helix in the N-terminus. A structural search and biochemical data suggest that SsoPDCD5 may function as a DNA-binding protein.

PubMed: 30713157
DOI: 10.1107/S2053230X18017673

実験手法

X-RAY DIFFRACTION (2.11 Å)