6INZ
Crystal structure of solute-binding protein complexed with unsaturated hyaluronan disaccharide
Summary for 6INZ
| Entry DOI | 10.2210/pdb6inz/pdb |
| Descriptor | Extracellular solute-binding protein family 1, 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | glycosaminoglycan, abc transporter, solute-binding protein, sugar binding protein |
| Biological source | Streptobacillus moniliformis DSM 12112 |
| Total number of polymer chains | 1 |
| Total formula weight | 58720.12 |
| Authors | Oiki, S.,Mikami, B.,Murata, K.,Hashimoto, W. (deposition date: 2018-10-29, release date: 2019-09-11, Last modification date: 2023-11-22) |
| Primary citation | Oiki, S.,Sato, M.,Mikami, B.,Murata, K.,Hashimoto, W. Substrate recognition by bacterial solute-binding protein is responsible for import of extracellular hyaluronan and chondroitin sulfate from the animal host. Biosci.Biotechnol.Biochem., 83:1946-1954, 2019 Cited by PubMed Abstract: Glycosaminoglycans (GAGs) such as hyaluronan and chondroitin in animal extracellular matrices contain disaccharide-repeating units. In a Gram-negative pathogenic , which belongs to Fusobacteria phylum and resides in rodent oral cavities, the solute-binding protein (Smon0123)-dependent ATP-binding cassette transporter imports unsaturated hyaluronan/chondroitin disaccharides into the cytoplasm after GAG lyase-dependent depolymerization. Here we show substrate recognition of unsaturated hyaluronan disaccharide by Smon0123. Moreover, Smon0123 exhibited no affinity for unsaturated chondroitin disaccharides containing three sulfate groups, distinct from non-sulfated, mono-sulfated, and di-sulfated chondroitin disaccharides previously identified as substrates. Crystal structure of Smon0123 with unsaturated hyaluronan disaccharide demonstrates that several residues, including Trp284 and Glu410, are crucial for binding to unsaturated hyaluronan/chondroitin disaccharides, whereas arrangements of water molecules at binding sites are found to be substrate dependent through comparison with substrate-bound structures determined previously. These residues are well conserved in Smon0123-like proteins of fusobacteria, and probably facilitate the fusobacterial residence in hyaluronan-rich oral cavities. PubMed: 31204616DOI: 10.1080/09168451.2019.1630250 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.289 Å) |
Structure validation
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