6INZ

Crystal structure of solute-binding protein complexed with unsaturated hyaluronan disaccharide

6INZ の概要

• エントリーDOI: 10.2210/pdb6inz/pdb
• 分子名称: Extracellular solute-binding protein family 1, 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: glycosaminoglycan, abc transporter, solute-binding protein, sugar binding protein
• 由来する生物種: Streptobacillus moniliformis DSM 12112
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58720.12

構造登録者

Oiki, S.,Mikami, B.,Murata, K.,Hashimoto, W. (登録日: 2018-10-29, 公開日: 2019-09-11, 最終更新日: 2023-11-22)

主引用文献

Oiki, S.,Sato, M.,Mikami, B.,Murata, K.,Hashimoto, W.
Substrate recognition by bacterial solute-binding protein is responsible for import of extracellular hyaluronan and chondroitin sulfate from the animal host.
Biosci.Biotechnol.Biochem., 83:1946-1954, 2019

PubMed Abstract: Glycosaminoglycans (GAGs) such as hyaluronan and chondroitin in animal extracellular matrices contain disaccharide-repeating units. In a Gram-negative pathogenic , which belongs to Fusobacteria phylum and resides in rodent oral cavities, the solute-binding protein (Smon0123)-dependent ATP-binding cassette transporter imports unsaturated hyaluronan/chondroitin disaccharides into the cytoplasm after GAG lyase-dependent depolymerization. Here we show substrate recognition of unsaturated hyaluronan disaccharide by Smon0123. Moreover, Smon0123 exhibited no affinity for unsaturated chondroitin disaccharides containing three sulfate groups, distinct from non-sulfated, mono-sulfated, and di-sulfated chondroitin disaccharides previously identified as substrates. Crystal structure of Smon0123 with unsaturated hyaluronan disaccharide demonstrates that several residues, including Trp284 and Glu410, are crucial for binding to unsaturated hyaluronan/chondroitin disaccharides, whereas arrangements of water molecules at binding sites are found to be substrate dependent through comparison with substrate-bound structures determined previously. These residues are well conserved in Smon0123-like proteins of fusobacteria, and probably facilitate the fusobacterial residence in hyaluronan-rich oral cavities.

PubMed: 31204616
DOI: 10.1080/09168451.2019.1630250

実験手法

X-RAY DIFFRACTION (2.289 Å)