6IHD

Crystal structure of Malate dehydrogenase from Metallosphaera sedula

6IHD の概要

• エントリーDOI: 10.2210/pdb6ihd/pdb
• 分子名称: Malate dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, 2-ETHOXYETHANOL, ... (4 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Metallosphaera sedula
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68206.09

構造登録者

Lee, D.,Kim, K.J. (登録日: 2018-09-29, 公開日: 2019-02-06, 最終更新日: 2023-11-22)

主引用文献

Lee, D.,Hong, J.,Kim, K.J.
Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula
Biochem. Biophys. Res. Commun., 509:833-838, 2019

PubMed Abstract: Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the central metabolism that converts malate to oxaloacetate. To elucidate the enzymatic properties of MDH from M. sedula (MsMDH), we determined the crystal structure of MsMDH as a complex with NAD and a ternary complex with malate and NAD. Based on its complex structures and biochemical experiments, we observed that MsMDH can utilize both NAD and NADP as a cofactor. In addition, we revealed that MsMDH shows a conformational change at the active site upon substrate binding. Based on the comparison with other MDHs, we revealed that MsMDH was distinguished from general MDHs due to a Lys80 residue, and this difference is likely to influence the unique cofactor specificity of MsMDH.

PubMed: 30638660
DOI: 10.1016/j.bbrc.2019.01.018

実験手法

X-RAY DIFFRACTION (2.3 Å)