6IGB

the structure of Pseudomonas aeruginosa Periplasmic gluconolactonase, PpgL

6IGB の概要

• エントリーDOI: 10.2210/pdb6igb/pdb
• 分子名称: Periplasmic gluconolactonase, PpgL, SULFATE ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: 6-phosphogluconolactonase activity, hydrolase
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78655.10

構造登録者

Song, Y.J.,Shen, Y.L.,Wang, K.L.,Li, T.,Zhu, Y.B.,Li, C.C.,He, L.H.,Zhao, N.L.,Zhao, C.,Yang, J.,Huang, Q.,Mu, X.Y. (登録日: 2018-09-25, 公開日: 2018-11-21, 最終更新日: 2023-11-22)

主引用文献

Song, Y.J.,Wang, K.L.,Shen, Y.L.,Gao, J.,Li, T.,Zhu, Y.B.,Li, C.C.,He, L.H.,Zhou, Q.X.,Zhao, N.L.,Zhao, C.,Yang, J.,Huang, Q.,Mu, X.Y.,Li, H.,Dou, D.F.,Liu, C.,He, J.H.,Sun, B.,Bao, R.
Structural and Functional Insights into PpgL, a Metal-Independent beta-Propeller Gluconolactonase That Contributes toPseudomonas aeruginosaVirulence.
Infect.Immun., 87:-, 2019

PubMed Abstract: Biofilm formation is a critical determinant in the pathopoiesis of It could significantly increase bacterial resistance to drugs and host defense. Thus, inhibition of biofilm matrix production could be regarded as a promising attempt to prevent colonization of and the subsequent infection. PpgL, a periplasmic gluconolactonase, has been reported to be involved in quorum-sensing (QS) system regulation. However, the detailed function and catalysis mechanism remain elusive. Here, the crystal structure of PpgL is described in the current study, along with biochemical analysis, revealing that PpgL is a typical β-propeller enzyme with unique metal-independent lactone hydrolysis activity. Consequently, comparative analysis of seven-bladed propeller lactone-catalyzing enzymes and mutagenesis studies identify the critical sites which contribute to the diverse catalytic and substrate recognition functions. In addition, the reduced biofilm formation and attenuated invasion phenotype resulting from deletion of confirm the importance of PpgL in pathogenesis. These results suggest that PpgL is a potential target for developing new agents against the diseases caused by .

PubMed: 30642898
DOI: 10.1128/IAI.00847-18

実験手法

X-RAY DIFFRACTION (1.651 Å)