6IFB

Structure of rhamnose bound beta-trefoil lectin from Entamoeba histolytica

6IFB の概要

• エントリーDOI: 10.2210/pdb6ifb/pdb
• 分子名称: lectin, beta-L-rhamnopyranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: beta-trefoil, lectin, entamoeba histolytica, rhamnose, sugar binding protein
• 由来する生物種: Entamoeba histolytica HM-1:IMSS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31319.08

構造登録者

Suguna, K.,Khan, F. (登録日: 2018-09-19, 公開日: 2019-09-25, 最終更新日: 2024-03-27)

主引用文献

Khan, F.,Kurre, D.,Suguna, K.
Crystal structures of a beta-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulfide bond-mediated dimeric forms.
Glycobiology, 30:474-488, 2020

PubMed Abstract: β-Trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophysical studies on a β-trefoil lectin from Entamoeba histolytica (EntTref), which exists as a mixture of monomers and dimers in solution. Further, we have determined the affinities of EntTref for rhamnose, galactose and different galactose-linked sugars. We obtained the crystal structure of EntTref in a sugar-free form (EntTref_apo) and a rhamnose-bound form (EntTref_rham). A novel Cys residue-mediated dimerization was revealed in the crystal structure of EntTref_apo while the structure of EntTref_rham provided the structural basis for the recognition of rhamnose by a β-trefoil lectin for the first time. To the best of our knowledge, this is the only report of the structural, functional and biophysical characterization of a β-trefoil lectin from a protozoan source and the first report of Cys-mediated dimerization in this class of lectins.

PubMed: 31967310
DOI: 10.1093/glycob/cwaa001

実験手法

X-RAY DIFFRACTION (1.37 Å)