6IEQ

Crystal Structure of HIV-1 Env ConM SOSIP.v7 in Complex with bNAb PGT124 and 35O22

Summary for 6IEQ

• Entry DOI: 10.2210/pdb6ieq/pdb
• Related PRD ID: PRD_900118
• Descriptor: Envelope glycoprotein gp160, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose, ... (14 entities in total)
• Functional Keywords: hiv-1, envelop protein, consensus sequence-based conm sosip, broadly neutralizing antibody, viral protein
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 6
• Total formula weight: 180561.42

Authors

Han, B.W.,Wilson, I.A. (deposition date: 2018-09-16, release date: 2019-05-22, Last modification date: 2024-10-23)

Primary citation

Sliepen, K.,Han, B.W.,Bontjer, I.,Mooij, P.,Garces, F.,Behrens, A.J.,Rantalainen, K.,Kumar, S.,Sarkar, A.,Brouwer, P.J.M.,Hua, Y.,Tolazzi, M.,Schermer, E.,Torres, J.L.,Ozorowski, G.,van der Woude, P.,de la Pena, A.T.,van Breemen, M.J.,Camacho-Sanchez, J.M.,Burger, J.A.,Medina-Ramirez, M.,Gonzalez, N.,Alcami, J.,LaBranche, C.,Scarlatti, G.,van Gils, M.J.,Crispin, M.,Montefiori, D.C.,Ward, A.B.,Koopman, G.,Moore, J.P.,Shattock, R.J.,Bogers, W.M.,Wilson, I.A.,Sanders, R.W.
Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence.
Nat Commun, 10:2355-2355, 2019

PubMed Abstract: Stabilized HIV-1 envelope glycoproteins (Env) that resemble the native Env are utilized in vaccination strategies aimed at inducing broadly neutralizing antibodies (bNAbs). To limit the exposure of rare isolate-specific antigenic residues/determinants we generated a SOSIP trimer based on a consensus sequence of all HIV-1 group M isolates (ConM). The ConM trimer displays the epitopes of most known bNAbs and several germline bNAb precursors. The crystal structure of the ConM trimer at 3.9 Å resolution resembles that of the native Env trimer and its antigenic surface displays few rare residues. The ConM trimer elicits strong NAb responses against the autologous virus in rabbits and macaques that are significantly enhanced when it is presented on ferritin nanoparticles. The dominant NAb specificity is directed against an epitope at or close to the trimer apex. Immunogens based on consensus sequences might have utility in engineering vaccines against HIV-1 and other viruses.

PubMed: 31142746
DOI: 10.1038/s41467-019-10262-5

Experimental method

X-RAY DIFFRACTION (3.9 Å)