6IEQ

Crystal Structure of HIV-1 Env ConM SOSIP.v7 in Complex with bNAb PGT124 and 35O22

6IEQ の概要

• エントリーDOI: 10.2210/pdb6ieq/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900118
• 分子名称: Envelope glycoprotein gp160, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose, ... (14 entities in total)
• 機能のキーワード: hiv-1, envelop protein, consensus sequence-based conm sosip, broadly neutralizing antibody, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 180561.42

構造登録者

Han, B.W.,Wilson, I.A. (登録日: 2018-09-16, 公開日: 2019-05-22, 最終更新日: 2024-10-23)

主引用文献

Sliepen, K.,Han, B.W.,Bontjer, I.,Mooij, P.,Garces, F.,Behrens, A.J.,Rantalainen, K.,Kumar, S.,Sarkar, A.,Brouwer, P.J.M.,Hua, Y.,Tolazzi, M.,Schermer, E.,Torres, J.L.,Ozorowski, G.,van der Woude, P.,de la Pena, A.T.,van Breemen, M.J.,Camacho-Sanchez, J.M.,Burger, J.A.,Medina-Ramirez, M.,Gonzalez, N.,Alcami, J.,LaBranche, C.,Scarlatti, G.,van Gils, M.J.,Crispin, M.,Montefiori, D.C.,Ward, A.B.,Koopman, G.,Moore, J.P.,Shattock, R.J.,Bogers, W.M.,Wilson, I.A.,Sanders, R.W.
Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence.
Nat Commun, 10:2355-2355, 2019

PubMed Abstract: Stabilized HIV-1 envelope glycoproteins (Env) that resemble the native Env are utilized in vaccination strategies aimed at inducing broadly neutralizing antibodies (bNAbs). To limit the exposure of rare isolate-specific antigenic residues/determinants we generated a SOSIP trimer based on a consensus sequence of all HIV-1 group M isolates (ConM). The ConM trimer displays the epitopes of most known bNAbs and several germline bNAb precursors. The crystal structure of the ConM trimer at 3.9 Å resolution resembles that of the native Env trimer and its antigenic surface displays few rare residues. The ConM trimer elicits strong NAb responses against the autologous virus in rabbits and macaques that are significantly enhanced when it is presented on ferritin nanoparticles. The dominant NAb specificity is directed against an epitope at or close to the trimer apex. Immunogens based on consensus sequences might have utility in engineering vaccines against HIV-1 and other viruses.

PubMed: 31142746
DOI: 10.1038/s41467-019-10262-5

実験手法

X-RAY DIFFRACTION (3.9 Å)