6IEO

Crystal structure of Mycobacterium tuberculosis HtrA1 (Rv1223) in regulated conformation

6IEO の概要

• エントリーDOI: 10.2210/pdb6ieo/pdb
• 分子名称: Probable serine protease HtrA (DEGP protein), phenylmethanesulfonic acid (3 entities in total)
• 機能のキーワード: hydrolase, htra family of serine protease, chymotrypsin like, periplasm, protein quality control and signal transduction, membrane protein
• 由来する生物種: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32694.59

構造登録者

Gupta, A.K.,Gopal, B. (登録日: 2018-09-14, 公開日: 2018-12-19, 最終更新日: 2024-10-30)

主引用文献

Gupta, A.K.,Behera, D.,Gopal, B.
The crystal structure of Mycobacterium tuberculosis high-temperature requirement A protein reveals an autoregulatory mechanism.
Acta Crystallogr F Struct Biol Commun, 74:803-809, 2018

PubMed Abstract: The crystal structure of Mycobacterium tuberculosis high-temperature requirement A (HtrA) protein was determined at 1.83 Å resolution. This membrane-associated protease is essential for the survival of M. tuberculosis. The crystal structure reveals that interactions between the PDZ domain and the catalytic domain in HtrA lead to an inactive conformation. This finding is consistent with its proposed role as a regulatory protease that is conditionally activated upon appropriate environmental triggers. The structure provides a basis for directed studies to evaluate the role of this essential protein and the regulatory pathways that are influenced by this protease.

PubMed: 30511675
DOI: 10.1107/S2053230X18016217

実験手法

X-RAY DIFFRACTION (1.83 Å)