6IEB

Structure of RVFV Gn and human monoclonal antibody R15

6IEB の概要

• エントリーDOI: 10.2210/pdb6ieb/pdb
• 分子名称: R15 H chain, R15 L chain, NSmGnGc, ... (4 entities in total)
• 機能のキーワード: rvfv, antibody, structural protein, structural protein-immune system complex, structural protein/immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 160198.23

構造登録者

Wang, Q.H.,Wu, Y.,Gao, F.,Qi, J.X.,Gao, G.F. (登録日: 2018-09-13, 公開日: 2019-04-10, 最終更新日: 2024-10-30)

主引用文献

Wang, Q.,Ma, T.,Wu, Y.,Chen, Z.,Zeng, H.,Tong, Z.,Gao, F.,Qi, J.,Zhao, Z.,Chai, Y.,Yang, H.,Wong, G.,Bi, Y.,Wu, L.,Shi, R.,Yang, M.,Song, J.,Jiang, H.,An, Z.,Wang, J.,Yilma, T.D.,Shi, Y.,Liu, W.J.,Liang, M.,Qin, C.,Gao, G.F.,Yan, J.
Neutralization mechanism of human monoclonal antibodies against Rift Valley fever virus.
Nat Microbiol, 4:1231-1241, 2019

PubMed Abstract: Rift Valley fever virus (RVFV) is a mosquito-borne pathogen that causes substantial morbidity and mortality in livestock and humans. To date, there are no licensed human vaccines or therapeutics available. Here, we report the isolation of monoclonal antibodies from a convalescent patient, targeting the RVFV envelope proteins Gn and Gc. The Gn-specific monoclonal antibodies exhibited much higher neutralizing activities in vitro and protection efficacies in mice against RVFV infection, compared to the Gc-specific monoclonal antibodies. The Gn monoclonal antibodies were found to interfere with soluble Gn binding to cells and prevent infection by blocking the attachment of virions to host cells. Structural analysis of Gn complexed with four Gn-specific monoclonal antibodies resulted in the definition of three antigenic patches (A, B and C) on Gn domain I. Both patches A and B are major neutralizing epitopes. Our results highlight the potential of antibody-based therapeutics and provide a structure-based rationale for designing vaccines against RVFV.

PubMed: 30936489
DOI: 10.1038/s41564-019-0411-z

実験手法

X-RAY DIFFRACTION (2.409 Å)