6ID4

Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01

6ID4 の概要

• エントリーDOI: 10.2210/pdb6id4/pdb
• 分子名称: MHC class I antigen, Beta-2-microglobulin, Heavy chain, ... (8 entities in total)
• 機能のキーワード: immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 185292.42

構造登録者

Lescar, J.,Wong, Y.H.,Liew, C.W.,Gu, Y.,MacAry, P.A. (登録日: 2018-09-08, 公開日: 2019-02-06, 最終更新日: 2024-10-23)

主引用文献

Gu, Y.,Wong, Y.H.,Liew, C.W.,Chan, C.E.Z.,Murali, T.M.,Yap, J.,Too, C.T.,Purushotorman, K.,Hamidinia, M.,El Sahili, A.,Goh, A.T.H.,Teo, R.Z.C.,Wood, K.J.,Hanson, B.J.,Gascoigne, N.R.J.,Lescar, J.,Vathsala, A.,MacAry, P.A.
Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
Nat Commun, 10:893-893, 2019

PubMed Abstract: Our understanding of the conformational and electrostatic determinants that underlie targeting of human leukocyte antigens (HLA) by anti-HLA alloantibodies is principally based upon in silico modelling. Here we provide a biochemical/biophysical and functional characterization of a human monoclonal alloantibody specific for a common HLA type, HLA-A*11:01. We present a 2.4 Å resolution map of the binding interface of this antibody on HLA-A*11:01 and compare the structural determinants with those utilized by T-cell receptor (TCR), killer-cell immunoglobulin-like receptor (KIR) and CD8 on the same molecule. These data provide a mechanistic insight into the paratope-epitope relationship between an alloantibody and its target HLA molecule in a biological context where other immune receptors are concomitantly engaged. This has important implications for our interpretation of serologic binding patterns of anti-HLA antibodies in sensitized individuals and thus, for the biology of human alloresponses.

PubMed: 30792391
DOI: 10.1038/s41467-019-08790-1

実験手法

X-RAY DIFFRACTION (2.4 Å)