6IB8

Structure of a complex of SuhB and NusA AR2 domain

6IB8 の概要

• エントリーDOI: 10.2210/pdb6ib8/pdb
• 分子名称: Inositol-1-monophosphatase, Transcription termination/antitermination protein NusA, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: suhb, nusa ar2 domain, antitermiantion, transcription
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 68240.78

構造登録者

Huang, Y.H.,Loll, B.,Wahl, M.C. (登録日: 2018-11-29, 公開日: 2019-04-17, 最終更新日: 2024-01-24)

主引用文献

Huang, Y.H.,Said, N.,Loll, B.,Wahl, M.C.
Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis.
Nucleic Acids Res., 47:6488-6503, 2019

PubMed Abstract: Ribosomal RNA synthesis in Escherichia coli involves a transcription complex, in which RNA polymerase is modified by a signal element on the transcript, Nus factors A, B, E and G, ribosomal protein S4 and inositol mono-phosphatase SuhB. This complex is resistant to ρ-dependent termination and facilitates ribosomal RNA folding, maturation and subunit assembly. The functional contributions of SuhB and their structural bases are presently unclear. We show that SuhB directly binds the RNA signal element and the C-terminal AR2 domain of NusA, and we delineate the atomic basis of the latter interaction by macromolecular crystallography. SuhB recruitment to a ribosomal RNA transcription complex depends on the RNA signal element but not on the NusA AR2 domain. SuhB in turn is required for stable integration of the NusB/E dimer into the complex. In vitro transcription assays revealed that SuhB is crucial for delaying or suppressing ρ-dependent termination, that SuhB also can reduce intrinsic termination, and that SuhB-AR2 contacts contribute to these effects. Together, our results reveal functions of SuhB during ribosomal RNA synthesis and delineate some of the underlying molecular interactions.

PubMed: 31020314
DOI: 10.1093/nar/gkz290

実験手法

X-RAY DIFFRACTION (1.646 Å)