6I9Q

Structure of the mouse CD98 heavy chain ectodomain

6I9Q の概要

• エントリーDOI: 10.2210/pdb6i9q/pdb
• 分子名称: 4F2 cell-surface antigen heavy chain, 1,2-ETHANEDIOL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: cd98hc, 4f2hc, amino acid transport, cd98 light chain, lat-1, lat-2, integrin beta subunit binding, single-pass type ii membrane protein, membrane protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48526.16

構造登録者

Schiefner, A.,Deuschle, F.-C.,Skerra, A. (登録日: 2018-11-24, 公開日: 2019-04-17, 最終更新日: 2024-01-24)

主引用文献

Deuschle, F.C.,Schiefner, A.,Skerra, A.
Structural differences between the ectodomains of murine and human CD98hc.
Proteins, 87:693-698, 2019

PubMed Abstract: The CD98 heavy chain (CD98hc) constitutes both a promising cell surface target for the treatment of cancers and a transcytosis receptor potentially useful for the brain delivery of therapeutics. However, pharmacokinetic studies and safety assessment of cognate antibodies or nonimmunoglobulin binding proteins in rodents is hampered by cross-species variability of both amino acid sequence and glycosylation pattern. Here, we report the crystal structure of the murine CD98hc extracellular domain and a comprehensive comparison with its human ortholog, revealing only one conserved surface patch that is neither shielded by glycosylation nor by the cell membrane with an accessible surface area typical for an antibody epitope. Our results imply the necessity of a surrogate approach for CD98hc-specific binding proteins with predictive power for clinical investigations.

PubMed: 30958588
DOI: 10.1002/prot.25686

実験手法

X-RAY DIFFRACTION (2.1 Å)