6I9K

Crystal structure of Jumping Spider Rhodopsin-1 bound to 9-cis retinal

Summary for 6I9K

• Entry DOI: 10.2210/pdb6i9k/pdb
• Descriptor: Kumopsin1, RETINAL, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (4 entities in total)
• Functional Keywords: rhodopsin, gpcr, light-sensitive, retinal, membrane protein
• Biological source: Hasarius adansoni
• Total number of polymer chains: 1
• Total formula weight: 46802.67

Authors

Varma, N.,Mutt, E.,Muehle, J.,Panneels, V.,Terakita, A.,Deupi, X.,Nogly, P.,Schertler, F.X.G.,Lesca, E. (deposition date: 2018-11-23, release date: 2019-07-03, Last modification date: 2024-10-23)

Primary citation

Varma, N.,Mutt, E.,Muhle, J.,Panneels, V.,Terakita, A.,Deupi, X.,Nogly, P.,Schertler, G.F.X.,Lesca, E.
Crystal structure of jumping spider rhodopsin-1 as a light sensitive GPCR.
Proc.Natl.Acad.Sci.USA, 116:14547-14556, 2019

PubMed Abstract: Light-sensitive G protein-coupled receptors (GPCRs)-rhodopsins-absorb photons to isomerize their covalently bound retinal, triggering conformational changes that result in downstream signaling cascades. Monostable rhodopsins release retinal upon isomerization as opposed to the retinal in bistable rhodopsins that "reisomerize" upon absorption of a second photon. Understanding the mechanistic differences between these light-sensitive GPCRs has been hindered by the scarcity of recombinant models of the latter. Here, we reveal the high-resolution crystal structure of a recombinant bistable rhodopsin, jumping spider rhodopsin-1, bound to the inverse agonist 9- retinal. We observe a water-mediated network around the ligand hinting toward the basis of their bistable nature. In contrast to bovine rhodopsin (monostable), the transmembrane bundle of jumping spider rhodopsin-1 as well that of the bistable squid rhodopsin adopts a more "activation-ready" conformation often observed in other nonphotosensitive class A GPCRs. These similarities suggest the role of jumping spider rhodopsin-1 as a potential model system in the study of the structure-function relationship of both photosensitive and nonphotosensitive class A GPCRs.

PubMed: 31249143
DOI: 10.1073/pnas.1902192116

Experimental method

X-RAY DIFFRACTION (2.145 Å)