6I84

Structure of transcribing RNA polymerase II-nucleosome complex

6I84 の概要

• エントリーDOI: 10.2210/pdb6i84/pdb
• EMDBエントリー: 4429
• 分子名称: Histone H3.2, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerases I, II, and III subunit RPABC1, ... (21 entities in total)
• 機能のキーワード: nucleosome, elongation, chromatin, rna polymerase ii, transcription
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 732091.00

構造登録者

Farnung, L.,Vos, S.M.,Cramer, P. (登録日: 2018-11-19, 公開日: 2019-01-02, 最終更新日: 2024-05-15)

主引用文献

Farnung, L.,Vos, S.M.,Cramer, P.
Structure of transcribing RNA polymerase II-nucleosome complex.
Nat Commun, 9:5432-5432, 2018

PubMed Abstract: Transcription of eukaryotic protein-coding genes requires passage of RNA polymerase II (Pol II) through nucleosomes, but it is unclear how this is achieved. Here we report the cryo-EM structure of transcribing Saccharomyces cerevisiae Pol II engaged with a downstream nucleosome core particle at an overall resolution of 4.4 Å. Pol II and the nucleosome are observed in a defined relative orientation that is not predicted. Pol II contacts both sides of the nucleosome dyad using its clamp head and lobe domains. Structural comparisons reveal that the elongation factors TFIIS, DSIF, NELF, SPT6, and PAF1 complex can be accommodated on the Pol II surface in the presence of the oriented nucleosome. Our results provide a starting point for analysing the mechanisms of chromatin transcription.

PubMed: 30575770
DOI: 10.1038/s41467-018-07870-y

実験手法

ELECTRON MICROSCOPY (4.4 Å)