Summary for 6I7V
| Entry DOI | 10.2210/pdb6i7v/pdb |
| Descriptor | 16S ribosomal RNA, 30S ribosomal protein S6, 30S ribosomal protein S7, ... (69 entities in total) |
| Functional Keywords | protein biosynthesis, ribosomes, paralog, rna, peptidyl, 30s, 70s, 16s, ribosomal subunit, ribosome |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 105 |
| Total formula weight | 4257119.69 |
| Authors | Pulk, A.,Cate, J.H.D.,Remme, J.,Lilleorg, S.,Reier, K.,Peil, L.,Liiv, A.,Tammsalu, T. (deposition date: 2018-11-19, release date: 2018-12-05, Last modification date: 2024-04-24) |
| Primary citation | Lilleorg, S.,Reier, K.,Pulk, A.,Liiv, A.,Tammsalu, T.,Peil, L.,Cate, J.D.,Remme, J. Bacterial ribosome heterogeneity: Changes in ribosomal protein composition during transition into stationary growth phase. Biochimie, 156:169-180, 2018 Cited by PubMed Abstract: Ribosomes consist of many small proteins and few large RNA molecules. Both components are necessary for ribosome functioning during translation. According to widely accepted view, bacterial ribosomes contain always the same complement of ribosomal proteins. Comparative bacterial genomics data indicates that several ribosomal proteins are encoded by multiple paralogous genes suggesting structural heterogeneity of ribosomes. In E. coli, two r-proteins bL31 and bL36 are encoded by two genes: rpmE and ykgM encode bL31 protein paralogs bL31A and bL31B, and rpmJ and ykgO encode bL36 protein paralogs bL36A and bL36B respectively. We have found several similarities and differences between ribosomes of exponential and stationary growth phases by using quantitative mass spectrometry and X-ray crystallography. First, composition of ribosome associating proteins changes profoundly as cells transition from exponential to stationary growth phase. Ribosomal core proteins bL31A and bL36A are replaced by bL31B and bL36B, respectively. Second, our X-ray structure of the 70S ribosome demonstrates that bL31B and bL36B proteins have similar ribosome binding sites to their A counterparts. Third, ribosome subpopulations containing A or B paralogs existed simultaneously demonstrating that E. coli ribosomes are heterogeneous with respect to their paralogous ribosomal protein composition that changes via protein exchange. PubMed: 30359641DOI: 10.1016/j.biochi.2018.10.013 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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