6I7V

Ribosomal protein paralogs bL31 and bL36

これはPDB形式変換不可エントリーです。

6I7V の概要

• エントリーDOI: 10.2210/pdb6i7v/pdb
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S6, 30S ribosomal protein S7, ... (69 entities in total)
• 機能のキーワード: protein biosynthesis, ribosomes, paralog, rna, peptidyl, 30s, 70s, 16s, ribosomal subunit, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 105
• 化学式量合計: 4257119.69

構造登録者

Pulk, A.,Cate, J.H.D.,Remme, J.,Lilleorg, S.,Reier, K.,Peil, L.,Liiv, A.,Tammsalu, T. (登録日: 2018-11-19, 公開日: 2018-12-05, 最終更新日: 2024-04-24)

主引用文献

Lilleorg, S.,Reier, K.,Pulk, A.,Liiv, A.,Tammsalu, T.,Peil, L.,Cate, J.D.,Remme, J.
Bacterial ribosome heterogeneity: Changes in ribosomal protein composition during transition into stationary growth phase.
Biochimie, 156:169-180, 2018

PubMed Abstract: Ribosomes consist of many small proteins and few large RNA molecules. Both components are necessary for ribosome functioning during translation. According to widely accepted view, bacterial ribosomes contain always the same complement of ribosomal proteins. Comparative bacterial genomics data indicates that several ribosomal proteins are encoded by multiple paralogous genes suggesting structural heterogeneity of ribosomes. In E. coli, two r-proteins bL31 and bL36 are encoded by two genes: rpmE and ykgM encode bL31 protein paralogs bL31A and bL31B, and rpmJ and ykgO encode bL36 protein paralogs bL36A and bL36B respectively. We have found several similarities and differences between ribosomes of exponential and stationary growth phases by using quantitative mass spectrometry and X-ray crystallography. First, composition of ribosome associating proteins changes profoundly as cells transition from exponential to stationary growth phase. Ribosomal core proteins bL31A and bL36A are replaced by bL31B and bL36B, respectively. Second, our X-ray structure of the 70S ribosome demonstrates that bL31B and bL36B proteins have similar ribosome binding sites to their A counterparts. Third, ribosome subpopulations containing A or B paralogs existed simultaneously demonstrating that E. coli ribosomes are heterogeneous with respect to their paralogous ribosomal protein composition that changes via protein exchange.

PubMed: 30359641
DOI: 10.1016/j.biochi.2018.10.013

実験手法

X-RAY DIFFRACTION (2.9 Å)