6I6H

Crystal structure of the KDEL receptor in the peptide bound state

6I6H の概要

• エントリーDOI: 10.2210/pdb6i6h/pdb
• 関連するPDBエントリー: 6I6B
• 分子名称: ER lumen protein-retaining receptor 2, AEKDEL peptide, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (4 entities in total)
• 機能のキーワード: intracellular protein receptor, membrane protein, kdel, erd2
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28621.98

構造登録者

Braeuer, P.,Newstead, S. (登録日: 2018-11-15, 公開日: 2019-02-27, 最終更新日: 2024-01-24)

主引用文献

Brauer, P.,Parker, J.L.,Gerondopoulos, A.,Zimmermann, I.,Seeger, M.A.,Barr, F.A.,Newstead, S.
Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor.
Science, 363:1103-1107, 2019

PubMed Abstract: Selective export and retrieval of proteins between the endoplasmic reticulum (ER) and Golgi apparatus is indispensable for eukaryotic cell function. An essential step in the retrieval of ER luminal proteins from the Golgi is the pH-dependent recognition of a carboxyl-terminal Lys-Asp-Glu-Leu (KDEL) signal by the KDEL receptor. Here, we present crystal structures of the chicken KDEL receptor in the apo ER state, KDEL-bound Golgi state, and in complex with an antagonistic synthetic nanobody (sybody). These structures show a transporter-like architecture that undergoes conformational changes upon KDEL binding and reveal a pH-dependent interaction network crucial for recognition of the carboxyl terminus of the KDEL signal. Complementary in vitro binding and in vivo cell localization data explain how these features create a pH-dependent retrieval system in the secretory pathway.

PubMed: 30846601
DOI: 10.1126/science.aaw2859

実験手法

X-RAY DIFFRACTION (2 Å)