6I3T

Crystal structure of murine neuroglobin bound to CO at 40 K.

6I3T の概要

• エントリーDOI: 10.2210/pdb6i3t/pdb
• 分子名称: Neuroglobin, PROTOPORPHYRIN IX CONTAINING FE, CARBON MONOXIDE, ... (7 entities in total)
• 機能のキーワード: oxygen transport/storage, carbon monoxide, photodissociation, oxygen transport
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18098.12

構造登録者

Savino, C.,Montemiglio, L.C.,Ardiccioni, C.,Exertier, C. (登録日: 2018-11-07, 公開日: 2019-09-11, 最終更新日: 2024-01-24)

主引用文献

Ardiccioni, C.,Arcovito, A.,Della Longa, S.,van der Linden, P.,Bourgeois, D.,Weik, M.,Montemiglio, L.C.,Savino, C.,Avella, G.,Exertier, C.,Carpentier, P.,Prange, T.,Brunori, M.,Colloc'h, N.,Vallone, B.
Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments.
Iucrj, 6:832-842, 2019

PubMed Abstract: A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV-visible microspectroscopy , solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15-40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.

PubMed: 31576217
DOI: 10.1107/S2052252519008157

実験手法

X-RAY DIFFRACTION (2 Å)