6I2N

Helical RNA-bound Hantaan virus nucleocapsid

Summary for 6I2N

• Entry DOI: 10.2210/pdb6i2n/pdb
• EMDB information: 0333
• Descriptor: RNA (5'-R(P*UP*UP*U)-3'), Nucleoprotein (2 entities in total)
• Functional Keywords: nucleoprotein, nucleocapsid, rna-binding, viral protein
• Biological source: Hantaan orthohantavirus (Korean hemorrhagic fever virus); More
• Total number of polymer chains: 2
• Total formula weight: 49135.81

Authors

Arragain, B.,Reguera, J.,Desfosses, A.,Gutsche, I.,Schoehn, G.,Malet, H. (deposition date: 2018-11-01, release date: 2019-01-23, Last modification date: 2024-05-15)

Primary citation

Arragain, B.,Reguera, J.,Desfosses, A.,Gutsche, I.,Schoehn, G.,Malet, H.
High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms.
Elife, 8:-, 2019

PubMed Abstract: Negative-strand RNA viruses condense their genome into helical nucleocapsids that constitute essential templates for viral replication and transcription. The intrinsic flexibility of nucleocapsids usually prevents their full-length structural characterisation at high resolution. Here, we describe purification of full-length recombinant metastable helical nucleocapsid of Hantaan virus ( family, order) and determine its structure at 3.3 Å resolution by cryo-electron microscopy. The structure reveals the mechanisms of helical multimerisation via sub-domain exchanges between protomers and highlights nucleotide positions in a continuous positively charged groove compatible with viral genome binding. It uncovers key sites for future structure-based design of antivirals that are currently lacking to counteract life-threatening hantavirus infections. The structure also suggests a model of nucleoprotein-polymerase interaction that would enable replication and transcription solely upon local disruption of the nucleocapsid.

PubMed: 30638449
DOI: 10.7554/eLife.43075

Experimental method

ELECTRON MICROSCOPY (3.3 Å)