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6I25

Flavin Analogue Sheds Light on Light-Oxygen-Voltage Domain Mechanism

6I25 の概要
エントリーDOI10.2210/pdb6i25/pdb
分子名称Aureochrome1-like protein, CHLORIDE ION, MAGNESIUM ION, ... (6 entities in total)
機能のキーワードlov domain, fmn, dark grown, fluorescence, light sensing, transcription factor pas domain, ochronomas danica, transcription
由来する生物種Ochromonas danica
タンパク質・核酸の鎖数1
化学式量合計15985.33
構造登録者
Rizkallah, P.J.,Kalvaitis, M.E. (登録日: 2018-10-31, 公開日: 2019-06-19, 最終更新日: 2024-10-09)
主引用文献Kalvaitis, M.E.,Johnson, L.A.,Mart, R.J.,Rizkallah, P.,Allemann, R.K.
A Noncanonical Chromophore Reveals Structural Rearrangements of the Light-Oxygen-Voltage Domain upon Photoactivation.
Biochemistry, 58:2608-2616, 2019
Cited by
PubMed Abstract: Light-oxygen-voltage (LOV) domains are increasingly used to engineer photoresponsive biological systems. While the photochemical cycle is well documented, the allosteric mechanism by which formation of a cysteinyl-flavin adduct leads to activation is unclear. Via replacement of flavin mononucleotide (FMN) with 5-deazaflavin mononucleotide (5dFMN) in the Aureochrome1a (Au1a) transcription factor from Ochromonas danica, a thermally stable cysteinyl-5dFMN adduct was generated. High-resolution crystal structures (<2 Å) under different illumination conditions with either FMN or 5dFMN chromophores reveal three conformations of the highly conserved glutamine 293. An allosteric hydrogen bond network linking the chromophore via Gln293 to the auxiliary A'α helix is observed. With FMN, a "flip" of the Gln293 side chain occurs between dark and lit states. 5dFMN cannot hydrogen bond through the C5 position and proved to be unable to support Au1a domain dimerization. Under blue light, the Gln293 side chain instead "swings" away in a conformation distal to the chromophore and not previously observed in existing LOV domain structures. Together, the multiple side chain conformations of Gln293 and functional analysis of 5dFMN provide new insight into the structural requirements for LOV domain activation.
PubMed: 31082213
DOI: 10.1021/acs.biochem.9b00255
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.97 Å)
構造検証レポート
Validation report summary of 6i25
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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