6I0N

Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with denuded glycan ended in anhNAM

6I0N の概要

• エントリーDOI: 10.2210/pdb6i0n/pdb
• 分子名称: Endolytic peptidoglycan transglycosylase RlpA, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-N-acetyl-beta-muramic acid-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-(2-ACETYLAMINO-4-HYDROXY-6,8-DIOXA-BICYCLO[3.2.1]OCT-3-YLOXY)-PROPIONIC ACID, ... (4 entities in total)
• 機能のキーワード: lytic transglycosylase, septum, spor domain, cell division, divisome, murein, denuded glycan, cell cycle, anhnam
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9148.24

構造登録者

Alcorlo, M.,Hermoso, J.A. (登録日: 2018-10-26, 公開日: 2019-11-13, 最終更新日: 2024-01-24)

主引用文献

Alcorlo, M.,Dik, D.A.,De Benedetti, S.,Mahasenan, K.V.,Lee, M.,Dominguez-Gil, T.,Hesek, D.,Lastochkin, E.,Lopez, D.,Boggess, B.,Mobashery, S.,Hermoso, J.A.
Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division.
Nat Commun, 10:5567-5567, 2019

PubMed Abstract: SPOR domains are widely present in bacterial proteins that recognize cell-wall peptidoglycan strands stripped of the peptide stems. This type of peptidoglycan is enriched in the septal ring as a product of catalysis by cell-wall amidases that participate in the separation of daughter cells during cell division. Here, we document binding of synthetic denuded glycan ligands to the SPOR domain of the lytic transglycosylase RlpA from Pseudomonas aeruginosa (SPOR-RlpA) by mass spectrometry and structural analyses, and demonstrate that indeed the presence of peptide stems in the peptidoglycan abrogates binding. The crystal structures of the SPOR domain, in the apo state and in complex with different synthetic glycan ligands, provide insights into the molecular basis for recognition and delineate a conserved pattern in other SPOR domains. The biological and structural observations presented here are followed up by molecular-dynamics simulations and by exploration of the effect on binding of distinct peptidoglycan modifications.

PubMed: 31804467
DOI: 10.1038/s41467-019-13354-4

実験手法

X-RAY DIFFRACTION (1.4 Å)