6HVR

Yeast 20S proteasome with human beta2i (1-53) in complex with 16

6HVR の概要

• エントリーDOI: 10.2210/pdb6hvr/pdb
• 関連するPDBエントリー: 5CZ4
• 分子名称: Proteasome subunit alpha type-2, Proteasome subunit beta type-4, Proteasome subunit beta type-5, ... (18 entities in total)
• 機能のキーワード: proteasome, mutant, inhibitor, binding analysis, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 732549.73

構造登録者

Huber, E.M.,Groll, M. (登録日: 2018-10-11, 公開日: 2019-01-30, 最終更新日: 2024-10-16)

主引用文献

Xin, B.T.,Huber, E.M.,de Bruin, G.,Heinemeyer, W.,Maurits, E.,Espinal, C.,Du, Y.,Janssens, M.,Weyburne, E.S.,Kisselev, A.F.,Florea, B.I.,Driessen, C.,van der Marel, G.A.,Groll, M.,Overkleeft, H.S.
Structure-Based Design of Inhibitors Selective for Human Proteasome beta 2c or beta 2i Subunits.
J.Med.Chem., 62:1626-1642, 2019

PubMed Abstract: Subunit-selective proteasome inhibitors are valuable tools to assess the biological and medicinal relevance of individual proteasome active sites. Whereas the inhibitors for the β1c, β1i, β5c, and β5i subunits exploit the differences in the substrate-binding channels identified by X-ray crystallography, compounds selectively targeting β2c or β2i could not yet be rationally designed because of the high structural similarity of these two subunits. Here, we report the development, chemical synthesis, and biological screening of a compound library that led to the identification of the β2c- and β2i-selective compounds LU-002c (4; IC β2c: 8 nM, IC β2i/β2c: 40-fold) and LU-002i (5; IC β2i: 220 nM, IC β2c/β2i: 45-fold), respectively. Co-crystal structures with β2 humanized yeast proteasomes visualize protein-ligand interactions crucial for subunit specificity. Altogether, organic syntheses, activity-based protein profiling, yeast mutagenesis, and structural biology allowed us to decipher significant differences of β2 substrate-binding channels and to complete the set of subunit-selective proteasome inhibitors.

PubMed: 30657666
DOI: 10.1021/acs.jmedchem.8b01884

実験手法

X-RAY DIFFRACTION (2.7 Å)