6HUZ

HmdII from Desulfurobacterium thermolithotrophum reconstituted with Fe-guanylylpyridinol (FeGP) cofactor and co-crystallized with methenyl-tetrahydrofolate form B

6HUZ の概要

• エントリーDOI: 10.2210/pdb6huz/pdb
• 分子名称: Coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin reductase-related protein, iron-guanylyl pyridinol cofactor, 5,10-Methenyltetrahydrofolate, ... (7 entities in total)
• 機能のキーワード: hydrogenase, h2-activation, lateral gene-transfer, cofactor biosynthesis, tetrahydromethanopterin, tetrahydrofolate, paralog, sulfur-reducing bacteria, metalloenzyme, oxidoreductase
• 由来する生物種: Desulfurobacterium thermolithotrophum DSM 11699
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42451.98

構造登録者

Watanabe, T.,Wagner, T.,Huang, G.,Kahnt, J.,Ataka, K.,Ermler, U.,Shima, S. (登録日: 2018-10-09, 公開日: 2019-01-09, 最終更新日: 2024-01-24)

主引用文献

Watanabe, T.,Wagner, T.,Huang, G.,Kahnt, J.,Ataka, K.,Ermler, U.,Shima, S.
The Bacterial [Fe]-Hydrogenase Paralog HmdII Uses Tetrahydrofolate Derivatives as Substrates.
Angew. Chem. Int. Ed. Engl., 58:3506-3510, 2019

PubMed Abstract: [Fe]-hydrogenase (Hmd) catalyzes the reversible hydrogenation of methenyl-tetrahydromethanopterin (methenyl-H MPT ) with H . H MPT is a C1-carrier of methanogenic archaea. One bacterial genus, Desulfurobacterium, contains putative genes for the Hmd paralog, termed HmdII, and the HcgA-G proteins. The latter are required for the biosynthesis of the prosthetic group of Hmd, the iron-guanylylpyridinol (FeGP) cofactor. This finding is intriguing because Hmd and HmdII strictly use H MPT derivatives that are absent in most bacteria. We identified the presence of the FeGP cofactor in D. thermolithotrophum. The bacterial HmdII reconstituted with the FeGP cofactor catalyzed the hydrogenation of derivatives of tetrahydrofolate, the bacterial C1-carrier, albeit with low enzymatic activities. The crystal structures show how Hmd recognizes tetrahydrofolate derivatives. These findings have an impact on future biotechnology by identifying a bacterial Hmd paralog.

PubMed: 30600878
DOI: 10.1002/anie.201813465

実験手法

X-RAY DIFFRACTION (1.85 Å)