6HUX

HmdII from Methanocaldococcus jannaschii reconstitued with Fe-guanylylpyridinol (FeGP) cofactor and co-crystallized with methenyl-tetrahydromethanopterin at 2.5 A resolution

Summary for 6HUX

• Entry DOI: 10.2210/pdb6hux/pdb
• Descriptor: H(2)-forming methylenetetrahydromethanopterin dehydrogenase-related protein MJ1338, ACETATE ION, iron-guanylyl pyridinol cofactor, ... (10 entities in total)
• Functional Keywords: hydrogenase, h2-activation, lateral gene-transfer, cofactor biosynthesis, tetrahydromethanopterin, paralog, methanogen, metalloenzyme, oxidoreductase
• Biological source: Methanocaldococcus jannaschii DSM 2661 (Methanococcus jannaschii)
• Total number of polymer chains: 1
• Total formula weight: 43610.64

Authors

Watanabe, T.,Wagner, T.,Huang, G.,Kahnt, J.,Ataka, K.,Ermler, U.,Shima, S. (deposition date: 2018-10-09, release date: 2019-01-09, Last modification date: 2024-01-24)

Primary citation

Watanabe, T.,Wagner, T.,Huang, G.,Kahnt, J.,Ataka, K.,Ermler, U.,Shima, S.
The Bacterial [Fe]-Hydrogenase Paralog HmdII Uses Tetrahydrofolate Derivatives as Substrates.
Angew. Chem. Int. Ed. Engl., 58:3506-3510, 2019

PubMed Abstract: [Fe]-hydrogenase (Hmd) catalyzes the reversible hydrogenation of methenyl-tetrahydromethanopterin (methenyl-H MPT ) with H . H MPT is a C1-carrier of methanogenic archaea. One bacterial genus, Desulfurobacterium, contains putative genes for the Hmd paralog, termed HmdII, and the HcgA-G proteins. The latter are required for the biosynthesis of the prosthetic group of Hmd, the iron-guanylylpyridinol (FeGP) cofactor. This finding is intriguing because Hmd and HmdII strictly use H MPT derivatives that are absent in most bacteria. We identified the presence of the FeGP cofactor in D. thermolithotrophum. The bacterial HmdII reconstituted with the FeGP cofactor catalyzed the hydrogenation of derivatives of tetrahydrofolate, the bacterial C1-carrier, albeit with low enzymatic activities. The crystal structures show how Hmd recognizes tetrahydrofolate derivatives. These findings have an impact on future biotechnology by identifying a bacterial Hmd paralog.

PubMed: 30600878
DOI: 10.1002/anie.201813465

Experimental method

X-RAY DIFFRACTION (2.5 Å)