6HTV

Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M in complex with isomaltotetraose

Summary for 6HTV

• Entry DOI: 10.2210/pdb6htv/pdb
• Descriptor: Alternansucrase, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, CALCIUM ION (3 entities in total)
• Functional Keywords: dextransucrase, dextran, transferase
• Biological source: Leuconostoc citreum
• Total number of polymer chains: 1
• Total formula weight: 144683.13

Authors

Claverie, M.,Cioci, G.,Remaud-Simeon, M.,Moulis, C.,Lippens, G. (deposition date: 2018-10-04, release date: 2019-09-11, Last modification date: 2024-01-24)

Primary citation

Claverie, M.,Cioci, G.,Guionnet, M.,Schorghuber, J.,Lichtenecker, R.,Moulis, C.,Remaud-Simeon, M.,Lippens, G.
Futile Encounter Engineering of the DSR-M Dextransucrase Modifies the Resulting Polymer Length.
Biochemistry, 58:2853-2859, 2019

PubMed Abstract: The factors that define the resulting polymer length of distributive polymerases are poorly understood. Here, starting from the crystal structure of the dextransucrase DSR-M in complex with an isomaltotetraose, we define different anchoring points for the incoming acceptor. Mutation of one of these, Trp624, decreases the catalytic rate of the enzyme but equally skews the size distribution of the resulting dextran chains toward shorter chains. Nuclear magnetic resonance analysis shows that this mutation influences both the dynamics of the active site and the water accessibility. Monte Carlo simulation of the elongation process allows interpretation of these results in terms of enhanced futile encounters, whereby the less effective binding increases the pool of effective seeds for the dextran chains and thereby directly determines the length distribution of the final polymers.

PubMed: 31140266
DOI: 10.1021/acs.biochem.9b00373

Experimental method

X-RAY DIFFRACTION (3.9 Å)