6HSV

Engineered higher-order assembly of Cholera Toxin B subunits via the addition of C-terminal parallel coiled-coiled domains

Summary for 6HSV

DescriptorToxin B subunit, PHOSPHATE ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
Functional Keywordscholera toxin b, ctb, parallel coiled coils, higher order assembly, self assembly, designed protein, nanotube, toxin
Biological sourceVibrio cholerae
Total number of polymer chains20
Total molecular weight281394.02
Authors
Pearson, A.R.,Turnbull, W.B.,Ross, J.F.,Trinh, C.H.,Webb, M.E. (deposition date: 2018-10-01, release date: 2019-03-20, Last modification date: 2019-04-10)
Primary citation
Ross, J.F.,Wildsmith, G.C.,Johnson, M.,Hurdiss, D.L.,Hollingsworth, K.,Thompson, R.F.,Mosayebi, M.,Trinh, C.H.,Paci, E.,Pearson, A.R.,Webb, M.E.,Turnbull, W.B.
Directed Assembly of Homopentameric Cholera Toxin B-Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages.
J.Am.Chem.Soc., 141:5211-5219, 2019
PubMed: 30856321 (PDB entries with the same primary citation)
DOI: 10.1021/jacs.8b11480
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.45 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2134 0.2% 3.3% 9.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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Molmil generated image of 6hsv
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More Biological unit images

Molmil generated image of 6hsv
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Molmil generated image of 6hsv
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Molmil generated image of 6hsv
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (6hsv.pdb1.gz [4.34 MB])
Coordinate files for Biological unit (6hsv.pdb2.gz [182.8 KB])
Coordinate files for Biological unit (6hsv.pdb3.gz [193.21 KB])
Coordinate files for Biological unit (6hsv.pdb4.gz [198.85 KB])
Coordinate files for Biological unit (6hsv.pdb5.gz [185.16 KB])