6HPD

The structure of a beta-glucuronidase from glycoside hydrolase family 2

6HPD の概要

• エントリーDOI: 10.2210/pdb6hpd/pdb
• 分子名称: Beta-galactosidase (GH2), BROMIDE ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: gh2, glycoside hydrolase, glucuronidase, hydrolase
• 由来する生物種: Formosa agariphila KMM 3901
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 112959.53

構造登録者

Robb, C.S.,Gerlach, N.,Reisky, L.,Bornshoeru, U.,Hehemann, J.H. (登録日: 2018-09-20, 公開日: 2019-07-24, 最終更新日: 2024-01-24)

主引用文献

Reisky, L.,Prechoux, A.,Zuhlke, M.K.,Baumgen, M.,Robb, C.S.,Gerlach, N.,Roret, T.,Stanetty, C.,Larocque, R.,Michel, G.,Song, T.,Markert, S.,Unfried, F.,Mihovilovic, M.D.,Trautwein-Schult, A.,Becher, D.,Schweder, T.,Bornscheuer, U.T.,Hehemann, J.H.
A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan.
Nat.Chem.Biol., 15:803-812, 2019

PubMed Abstract: Marine seaweeds increasingly grow into extensive algal blooms, which are detrimental to coastal ecosystems, tourism and aquaculture. However, algal biomass is also emerging as a sustainable raw material for the bioeconomy. The potential exploitation of algae is hindered by our limited knowledge of the microbial pathways-and hence the distinct biochemical functions of the enzymes involved-that convert algal polysaccharides into oligo- and monosaccharides. Understanding these processes would be essential, however, for applications such as the fermentation of algal biomass into bioethanol or other value-added compounds. Here, we describe the metabolic pathway that enables the marine flavobacterium Formosa agariphila to degrade ulvan, the main cell wall polysaccharide of bloom-forming Ulva species. The pathway involves 12 biochemically characterized carbohydrate-active enzymes, including two polysaccharide lyases, three sulfatases and seven glycoside hydrolases that sequentially break down ulvan into fermentable monosaccharides. This way, the enzymes turn a previously unexploited renewable into a valuable and ecologically sustainable bioresource.

PubMed: 31285597
DOI: 10.1038/s41589-019-0311-9

実験手法

X-RAY DIFFRACTION (2.43 Å)