6HPC

Crystal structure of the HicB antitoxin from E. coli

6HPC の概要

• エントリーDOI: 10.2210/pdb6hpc/pdb
• 関連するPDBエントリー: 6HPB
• 分子名称: Antitoxin HicB (2 entities in total)
• 機能のキーワード: dna binding, helix-turn-helix, hth, antitoxin
• 由来する生物種: Escherichia coli 2-210-07_S3_C3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30898.27

構造登録者

Manav, M.C.,Brodersen, D.E. (登録日: 2018-09-20, 公開日: 2019-09-18, 最終更新日: 2024-10-16)

主引用文献

Manav, M.C.,Turnbull, K.J.,Jurenas, D.,Garcia-Pino, A.,Gerdes, K.,Brodersen, D.E.
The E. coli HicB Antitoxin Contains a Structurally Stable Helix-Turn-Helix DNA Binding Domain.
Structure, 27:1675-1685.e3, 2019

PubMed Abstract: The E. coli hicAB type II toxin-antitoxin locus is unusual by being controlled by two promoters and by having the toxin encoded upstream of the antitoxin. HicA toxins contain a double-stranded RNA-binding fold and cleaves both mRNA and tmRNA in vivo, while HicB antitoxins contain a partial RNase H fold and either a helix-turn-helix (HTH) or ribbon-helix-helix domain. It is not known how an HTH DNA-binding domain affects higher-order structure for the HicAB modules. Here, we present crystal structures of the isolated E. coli HicB antitoxin and full-length HicAB complex showing that HicB forms a stable DNA-binding module and interacts in a canonical way with HicA despite the presence of an HTH-type DNA-binding domain. No major structural rearrangements take place upon binding of the toxin. Both structures expose well-ordered DNA-binding motifs allowing a model for DNA binding by the antitoxin to be generated.

PubMed: 31495532
DOI: 10.1016/j.str.2019.08.008

実験手法

X-RAY DIFFRACTION (2.26 Å)