6HN7

Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.

6HN7 の概要

• エントリーDOI: 10.2210/pdb6hn7/pdb
• 分子名称: Redirecting phage packaging protein C (RppC), Terminase small subunit (3 entities in total)
• 機能のキーワード: redirecting packaging protein, dna binding protein, hetero-dimer, phage interference, protein complex
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29301.11

構造登録者

Penades, J.R.,Bacarizo, J.,Marina, A.,Alqasmi, M.,Fillol-Salom, A.,Roszak, A.W.,Ciges-Tomas, J.R. (登録日: 2018-09-14, 公開日: 2019-07-31, 最終更新日: 2024-01-24)

主引用文献

Fillol-Salom, A.,Bacarizo, J.,Alqasmi, M.,Ciges-Tomas, J.R.,Martinez-Rubio, R.,Roszak, A.W.,Cogdell, R.J.,Chen, J.,Marina, A.,Penades, J.R.
Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.
Mol.Cell, 75:1020-1030.e4, 2019

PubMed Abstract: Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and how they block phage reproduction remains to be determined. Using genetic and structural analyses, we solve the mystery here by showing that the Gram-negative PICIs encode a protein that simultaneously performs these processes. This protein, which we have named Rpp (for redirecting phage packaging), interacts with the phage terminase small subunit, forming a heterocomplex. This complex is unable to recognize the phage DNA, blocking phage packaging, but specifically binds to the PICI genome, promoting PICI packaging. Our studies reveal the mechanism of action that allows PICI dissemination in nature, introducing a new paradigm in the understanding of the biology of pathogenicity islands and therefore of bacterial pathogen evolution.

PubMed: 31350119
DOI: 10.1016/j.molcel.2019.06.017

実験手法

X-RAY DIFFRACTION (3 Å)