6HLE

X-ray structure of furin in complex with the P6-P2-cyclized peptide H-Lys-Arg-Arg-Tle-Lys-4-Amba

Summary for 6HLE

• Entry DOI: 10.2210/pdb6hle/pdb
• Descriptor: Furin, LYS-ARG-ARG-TBG-LYS-00S, CALCIUM ION, ... (9 entities in total)
• Functional Keywords: protease proprotein convertase inhibitor complex cyclic peptide, hydrolase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 53914.69

Authors

Dahms, S.O. (deposition date: 2018-09-11, release date: 2019-02-06, Last modification date: 2024-10-16)

Primary citation

Van Lam van, T.,Ivanova, T.,Hardes, K.,Heindl, M.R.,Morty, R.E.,Bottcher-Friebertshauser, E.,Lindberg, I.,Than, M.E.,Dahms, S.O.,Steinmetzer, T.
Design, Synthesis, and Characterization of Macrocyclic Inhibitors of the Proprotein Convertase Furin.
Chemmedchem, 14:673-685, 2019

PubMed Abstract: The activation of viral glycoproteins by the host protease furin is an essential step in the replication of numerous pathogenic viruses. Thus, effective inhibitors of furin could serve as broad-spectrum antiviral drugs. A crystal structure of an inhibitory hexapeptide derivative in complex with furin served as template for the rational design of various types of new cyclic inhibitors. Most of the prepared derivatives are relatively potent furin inhibitors with inhibition constants in the low nanomolar or even sub-nanomolar range. For seven derivatives the crystal structures in complex with furin could be determined. In three complexes, electron density was found for the entire inhibitor. In the other cases the structures could be determined only for the P6/P5-P1 segments, which directly interact with furin. The cyclic derivatives together with two non-cyclic reference compounds were tested as inhibitors of the proteolytic activation and replication of respiratory syncytial virus in cells. Significant antiviral activity was found for both linear reference inhibitors, whereas a negligible efficacy was determined for the cyclic derivatives.

PubMed: 30680958
DOI: 10.1002/cmdc.201800807

Experimental method

X-RAY DIFFRACTION (1.994 Å)