6HKT

Structure of an H1-bound 6-nucleosome array

6HKT の概要

• エントリーDOI: 10.2210/pdb6hkt/pdb
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: chromatin, nucleosome array, chromatin fiber, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 50
• 化学式量合計: 1366005.46

構造登録者

Garcia-Saez, I.,Dimitrov, S.,Petosa, C. (登録日: 2018-09-08, 公開日: 2018-10-31, 最終更新日: 2024-01-17)

主引用文献

Garcia-Saez, I.,Menoni, H.,Boopathi, R.,Shukla, M.S.,Soueidan, L.,Noirclerc-Savoye, M.,Le Roy, A.,Skoufias, D.A.,Bednar, J.,Hamiche, A.,Angelov, D.,Petosa, C.,Dimitrov, S.
Structure of an H1-Bound 6-Nucleosome Array Reveals an Untwisted Two-Start Chromatin Fiber Conformation.
Mol. Cell, 72:902-915.e7, 2018

PubMed Abstract: Chromatin adopts a diversity of regular and irregular fiber structures in vitro and in vivo. However, how an array of nucleosomes folds into and switches between different fiber conformations is poorly understood. We report the 9.7 Å resolution crystal structure of a 6-nucleosome array bound to linker histone H1 determined under ionic conditions that favor incomplete chromatin condensation. The structure reveals a flat two-start helix with uniform nucleosomal stacking interfaces and a nucleosome packing density that is only half that of a twisted 30-nm fiber. Hydroxyl radical footprinting indicates that H1 binds the array in an on-dyad configuration resembling that observed for mononucleosomes. Biophysical, cryo-EM, and crosslinking data validate the crystal structure and reveal that a minor change in ionic environment shifts the conformational landscape to a more compact, twisted form. These findings provide insights into the structural plasticity of chromatin and suggest a possible assembly pathway for a 30-nm fiber.

PubMed: 30392928
DOI: 10.1016/j.molcel.2018.09.027

実験手法

X-RAY DIFFRACTION (9.7 Å)