6HK1

Crystal structure of the Thiazole synthase from Methanothermococcus thermolithotrophicus co-crystallized with Tb-Xo4

Summary for 6HK1

• Entry DOI: 10.2210/pdb6hk1/pdb
• Descriptor: Thiazole synthase, TERBIUM(III) ION, [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3R)-2,3,5-tris(oxidanyl)-4-oxidanylidene-pentyl] hydrogen phosphate, ... (8 entities in total)
• Functional Keywords: native purification, de novo phasing, nucleating agent, crystallophore, tb-xo4, lanthanide complex, molecular glue, biosynthetic protein
• Biological source: Methanothermococcus thermolithotrophicus
• Total number of polymer chains: 6
• Total formula weight: 179440.29

Authors

Engilberge, S.,Wagner, T.,Santoni, G.,Breyton, C.,Shima, S.,Franzetti, B.,Riobe, F.,Maury, O.,Girard, E. (deposition date: 2018-09-05, release date: 2019-06-19, Last modification date: 2024-05-15)

Primary citation

Engilberge, S.,Wagner, T.,Santoni, G.,Breyton, C.,Shima, S.,Franzetti, B.,Riobe, F.,Maury, O.,Girard, E.
Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.
J.Appl.Crystallogr., 52:722-731, 2019

PubMed Abstract: Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches.

PubMed: 31396026
DOI: 10.1107/S1600576719006381

Experimental method

X-RAY DIFFRACTION (2.55 Å)