6HIP

Structure of SPF45 UHM bound to HIV-1 Rev ULM

6HIP の概要

• エントリーDOI: 10.2210/pdb6hip/pdb
• 分子名称: Splicing factor 45, HIV-1 Rev (41-49), TRYPTOPHAN, ... (5 entities in total)
• 機能のキーワード: splicing factor, viral protein, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 24898.71

構造登録者

Pabis, M.,Corsini, L.,Sattler, M. (登録日: 2018-08-30, 公開日: 2019-03-27, 最終更新日: 2024-01-17)

主引用文献

Pabis, M.,Corsini, L.,Vincendeau, M.,Tripsianes, K.,Gibson, T.J.,Brack-Werner, R.,Sattler, M.
Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors.
Nucleic Acids Res., 47:4859-4871, 2019

PubMed Abstract: The HIV-1 protein Rev is essential for virus replication and ensures the expression of partially spliced and unspliced transcripts. We identified a ULM (UHM ligand motif) motif in the Arginine-Rich Motif (ARM) of the Rev protein. ULMs (UHM ligand motif) mediate protein interactions during spliceosome assembly by binding to UHM (U2AF homology motifs) domains. Using NMR, biophysical methods and crystallography we show that the Rev ULM binds to the UHMs of U2AF65 and SPF45. The highly conserved Trp45 in the Rev ULM is crucial for UHM binding in vitro, for Rev co-precipitation with U2AF65 in human cells and for proper processing of HIV transcripts. Thus, Rev-ULM interactions with UHM splicing factors contribute to the regulation of HIV-1 transcript processing, also at the splicing level. The Rev ULM is an example of viral mimicry of host short linear motifs that enables the virus to interfere with the host molecular machinery.

PubMed: 30892606
DOI: 10.1093/nar/gkz185

実験手法

X-RAY DIFFRACTION (1.2 Å)