6HEC

PAN-proteasome in state 4

6HEC の概要

• エントリーDOI: 10.2210/pdb6hec/pdb
• EMDBエントリー: 0215
• 分子名称: Proteasome subunit alpha, Proteasome subunit beta, Proteasome-activating nucleotidase, ... (6 entities in total)
• 機能のキーワード: pan, proteasome, aaa-atpase, archaea, hydrolase
• 由来する生物種: Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126); 詳細
• タンパク質・核酸の鎖数: 34
• 化学式量合計: 957233.51

構造登録者

Majumder, P.,Rudack, T.,Beck, F.,Baumeister, W. (登録日: 2018-08-20, 公開日: 2018-12-26, 最終更新日: 2024-05-15)

主引用文献

Majumder, P.,Rudack, T.,Beck, F.,Danev, R.,Pfeifer, G.,Nagy, I.,Baumeister, W.
Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle.
Proc. Natl. Acad. Sci. U.S.A., 116:534-539, 2019

PubMed Abstract: Proteasomes occur in all three domains of life, and are the principal molecular machines for the regulated degradation of intracellular proteins. They play key roles in the maintenance of protein homeostasis, and control vital cellular processes. While the eukaryotic 26S proteasome is extensively characterized, its putative evolutionary precursor, the archaeal proteasome, remains poorly understood. The primordial archaeal proteasome consists of a 20S proteolytic core particle (CP), and an AAA-ATPase module. This minimal complex degrades protein unassisted by non-ATPase subunits that are present in a 26S proteasome regulatory particle (RP). Using cryo-EM single-particle analysis, we determined structures of the archaeal CP in complex with the AAA-ATPase PAN (proteasome-activating nucleotidase). Five conformational states were identified, elucidating the functional cycle of PAN, and its interaction with the CP. Coexisting nucleotide states, and correlated intersubunit signaling features, coordinate rotation of the PAN-ATPase staircase, and allosterically regulate N-domain motions and CP gate opening. These findings reveal the structural basis for a sequential around-the-ring ATPase cycle, which is likely conserved in AAA-ATPases.

PubMed: 30559193
DOI: 10.1073/pnas.1817752116

実験手法

ELECTRON MICROSCOPY (6.95 Å)