6HDS

Crystal Structure of apo short afifavidin

6HDS の概要

• エントリーDOI: 10.2210/pdb6hds/pdb
• 分子名称: short afifavidin (2 entities in total)
• 機能のキーワード: avidin, biotin, protein assembly, high affinity systems, dimeric avidins, biotin binding protein
• 由来する生物種: Afifella pfennigii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 55544.53

構造登録者

Livnah, O.,Avraham, O. (登録日: 2018-08-19, 公開日: 2018-11-14, 最終更新日: 2024-11-06)

主引用文献

Avraham, O.,Bayer, E.A.,Livnah, O.
Crystal structure of afifavidin reveals common features of molecular assemblage in the bacterial dimeric avidins.
FEBS J., 285:4617-4630, 2018

PubMed Abstract: The subfamily of bacterial dimeric avidins is being extended through the discovery of additional members originating from diverse sources. All of these newly discovered dimeric avidin forms exhibit high affinity towards biotin, despite their lack of critical Trp in the classical tetrameric forms. The common feature of forming cylinder-like multimers (hexamers and octamers) seems to be more than a random occurrence, which generally characterizes their apo forms in the crystalline state and also in some cases in solution. Afifavidin from the Gram-negative α-proteobacterium Afifella pfennigii is the fourth member of the subfamily of dimers, which, in the intact apo form, also congregates into octamers both in the solution and in the crystalline state, whereby the C-terminal extended segments stretch into the biotin-binding sites of adjacent non-canonical monomers. The intact apo afifavidin molecule self-assembles into toroid-shaped nanostructures that dissociate into the inherent dimers upon binding biotin. On removal of the C-terminal regions, the short-form of afifavidin forms dimers both in the solution and in the crystalline states. The high affinity of the dimeric forms of afifavidin towards biotin is maintained, due to the conserved disulfide bridge between L3,4 and L5,6 and the presence of Phe50 in L3,4 that compensate for the lack of the critical Trp in the tetrameric avidins. These cyclic multimeric-avidin assemblies may be exploited in the future to further diversify biotin-based nanotechnology or to serve as building blocks in the construction of bio-inspired materials. DATABASE: Structural data are available in the PDB databases under the accession numbers: 6HDV, 6HDS, 6HDT.

PubMed: 30369031
DOI: 10.1111/febs.14685

実験手法

X-RAY DIFFRACTION (1.74 Å)