6HCR

Synthetic Self-assembling ADDomer Platform for Highly Efficient Vaccination by Genetically-encoded Multi-epitope Display

これはPDB形式変換不可エントリーです。

6HCR の概要

• エントリーDOI: 10.2210/pdb6hcr/pdb
• EMDBエントリー: 0198
• 分子名称: Penton protein (1 entity in total)
• 機能のキーワード: engineered, virus, like, particle, addomer, ad3, penton, base, adenovirus, synthetic, vaccine, biobrick, human, homosapien, dodecahedron, icosohedral, epitode, display, scaffold, virus like particle
• 由来する生物種: Human adenovirus B serotype 3
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 3815897.58

構造登録者

Bufton, J.C.,Berger, I.,Schaffitzel, C.,Garzoni, F.,Rabi, F.A. (登録日: 2018-08-16, 公開日: 2019-08-28, 最終更新日: 2024-05-15)

主引用文献

Vragniau, C.,Bufton, J.C.,Garzoni, F.,Stermann, E.,Rabi, F.,Terrat, C.,Guidetti, M.,Josserand, V.,Williams, M.,Woods, C.J.,Viedma, G.,Bates, P.,Verrier, B.,Chaperot, L.,Schaffitzel, C.,Berger, I.,Fender, P.
Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display.
Sci Adv, 5:eaaw2853-eaaw2853, 2019

PubMed Abstract: Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo-electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

PubMed: 31620562
DOI: 10.1126/sciadv.aaw2853

実験手法

ELECTRON MICROSCOPY (3.5 Å)