6HCL

Crystal structure of a MFS transporter with Ligand at 2.69 Angstroem resolution

Summary for 6HCL

• Entry DOI: 10.2210/pdb6hcl/pdb
• Related: 6G9X
• Descriptor: Major facilitator superfamily MFS_1, (2S)-2-HYDROXYPROPANOIC ACID, nonyl beta-D-glucopyranoside (3 entities in total)
• Functional Keywords: mfs transporter, membrane protein
• Biological source: Syntrophobacter fumaroxidans MPOB
• Total number of polymer chains: 2
• Total formula weight: 90176.12

Authors

Kalbermatter, D.,Bosshart, P.,Bonetti, S.,Fotiadis, D. (deposition date: 2018-08-15, release date: 2019-07-03, Last modification date: 2024-01-17)

Primary citation

Bosshart, P.D.,Kalbermatter, D.,Bonetti, S.,Fotiadis, D.
Mechanistic basis of L-lactate transport in the SLC16 solute carrier family.
Nat Commun, 10:2649-2649, 2019

PubMed Abstract: In human and other mammalian cells, transport of L-lactate across plasma membranes is mainly catalyzed by monocarboxylate transporters (MCTs) of the SLC16 solute carrier family. MCTs play an important role in cancer metabolism and are promising targets for tumor treatment. Here, we report the crystal structures of an SLC16 family homologue with two different bound ligands at 2.54 and 2.69 Å resolution. The structures show the transporter in the pharmacologically relevant outward-open conformation. Structural information together with a detailed structure-based analysis of the transport function provide important insights into the molecular working mechanisms of ligand binding and L-lactate transport.

PubMed: 31201333
DOI: 10.1038/s41467-019-10566-6

Experimental method

X-RAY DIFFRACTION (2.5 Å)