6HBD

Crystal structure of MSMEG_1712 from Mycobacterium smegmatis in complex with Beta-D-Galactofuranose

6HBD の概要

• エントリーDOI: 10.2210/pdb6hbd/pdb
• 分子名称: ABC transporter periplasmic-binding protein YtfQ, ZINC ION, beta-D-galactofuranose, ... (4 entities in total)
• 機能のキーワード: periplasmic binding protein, sugar binding protein
• 由来する生物種: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69002.82

構造登録者

Li, M.,Mueller, C.,Einsle, O.,Jessen-Trefzer, C. (登録日: 2018-08-10, 公開日: 2019-05-01, 最終更新日: 2024-01-17)

主引用文献

Li, M.,Muller, C.,Frohlich, K.,Gorka, O.,Zhang, L.,Gross, O.,Schilling, O.,Einsle, O.,Jessen-Trefzer, C.
Detection and Characterization of a Mycobacterial L-Arabinofuranose ABC Transporter Identified with a Rapid Lipoproteomics Protocol.
Cell Chem Biol, 26:852-, 2019

PubMed Abstract: Nutrient uptake is essential for survival of organisms, and carbohydrates serve as a crucial carbon and energy source for most microorganisms. Given the importance of mycobacteria as human pathogens a detailed knowledge of carbohydrate uptake transporters is highly desirable, but currently available information is severely limited and mainly based on in silico analyses. Moreover, there is only very little data available on the in vitro characterization of carbohydrate transporters from mycobacterial species. To overcome these significant limitations there is a strong demand for innovative approaches to experimentally match substrates to ATP-binding cassette (ABC) transporters in a straightforward manner. Our study focuses on the model organism Mycobacterium smegmatis and identifies a mycobacterial ABC transport system based on a rapid label-free mass spectrometry lipoproteomics assay with broad applicability. Further validation and X-ray structure analyses reveal a highly selective mycobacterial L-arabinose uptake system.

PubMed: 31006617
DOI: 10.1016/j.chembiol.2019.03.002

実験手法

X-RAY DIFFRACTION (2.44 Å)