6H56

Effector domain of Pseudomonas aeruginosa VgrG2b

6H56 の概要

• エントリーDOI: 10.2210/pdb6h56/pdb
• 分子名称: Effector domain of Pseudomonas aeruginosa VgrG2b, ZINC ION (2 entities in total)
• 機能のキーワード: zn binding, t6ss, effector, metal binding protein
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55406.84

構造登録者

Forster, A.,Freemont, P.S.,Filloux, A. (登録日: 2018-07-23, 公開日: 2019-07-24, 最終更新日: 2024-05-15)

主引用文献

Wood, T.E.,Howard, S.A.,Forster, A.,Nolan, L.M.,Manoli, E.,Bullen, N.P.,Yau, H.C.L.,Hachani, A.,Hayward, R.D.,Whitney, J.C.,Vollmer, W.,Freemont, P.S.,Filloux, A.
The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology.
Cell Rep, 29:187-201.e7, 2019

PubMed Abstract: The type VI secretion system (T6SS) is crucial in interbacterial competition and is a virulence determinant of many Gram-negative bacteria. Several T6SS effectors are covalently fused to secreted T6SS structural components such as the VgrG spike for delivery into target cells. In Pseudomonas aeruginosa, the VgrG2b effector was previously proposed to mediate bacterial internalization into eukaryotic cells. In this work, we find that the VgrG2b C-terminal domain (VgrG2b) elicits toxicity in the bacterial periplasm, counteracted by a cognate immunity protein. We resolve the structure of VgrG2b and confirm it is a member of the zinc-metallopeptidase family of enzymes. We show that this effector causes membrane blebbing at midcell, which suggests a distinct type of T6SS-mediated growth inhibition through interference with cell division, mimicking the impact of β-lactam antibiotics. Our study introduces a further effector family to the T6SS arsenal and demonstrates that VgrG2b can target both prokaryotic and eukaryotic cells.

PubMed: 31577948
DOI: 10.1016/j.celrep.2019.08.094

実験手法

X-RAY DIFFRACTION (3.2 Å)