6H4C

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

6H4C の概要

• エントリーDOI: 10.2210/pdb6h4c/pdb
• 分子名称: dUTPase, Orf20, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: sapi, repressor, complex, structural protein
• 由来する生物種: Staphylococcus phage phi11 (Bacteriophage phi-11); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 157499.83

構造登録者

Ciges-Tomas, J.R.,Alite, C.,Bowring, J.Z.,Donderis, J.,Penades, J.R.,Marina, A. (登録日: 2018-07-20, 公開日: 2019-08-28, 最終更新日: 2024-05-15)

主引用文献

Rafael Ciges-Tomas, J.,Alite, C.,Humphrey, S.,Donderis, J.,Bowring, J.,Salvatella, X.,Penades, J.R.,Marina, A.
The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature.
Nat Commun, 10:3676-3676, 2019

PubMed Abstract: Stl is a master repressor encoded by Staphylococcus aureus pathogenicity islands (SaPIs) that maintains integration of these elements in the bacterial chromosome. After infection or induction of a resident helper phage, SaPIs are de-repressed by specific interactions of phage proteins with Stl. SaPIs have evolved a fascinating mechanism to ensure their promiscuous transfer by targeting structurally unrelated proteins performing identically conserved functions for the phage. Here we decipher the molecular mechanism of this elegant strategy by determining the structure of SaPIbov1 Stl alone and in complex with two structurally unrelated dUTPases from different S. aureus phages. Remarkably, SaPIbov1 Stl has evolved different domains implicated in DNA and partner recognition specificity. This work presents the solved structure of a SaPI repressor protein and the discovery of a modular repressor that acquires multispecificity through domain recruiting. Our results establish the mechanism that allows widespread dissemination of SaPIs in nature.

PubMed: 31417084
DOI: 10.1038/s41467-019-11504-2

実験手法

X-RAY DIFFRACTION (2.52 Å)