6H0T
Crystal structure of native recombinant human bile salt activated lipase
Summary for 6H0T
| Entry DOI | 10.2210/pdb6h0t/pdb |
| Descriptor | Bile salt-activated lipase, ZINC ION, ACETATE ION, ... (5 entities in total) |
| Functional Keywords | lipase, alpha-beta hydrolase., hydrolase |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 61594.26 |
| Authors | Touvrey, C.,Brazzolotto, X.,Nachon, F. (deposition date: 2018-07-10, release date: 2019-03-27, Last modification date: 2024-01-17) |
| Primary citation | Touvrey, C.,Courageux, C.,Guillon, V.,Terreux, R.,Nachon, F.,Brazzolotto, X. X-ray structures of human bile-salt activated lipase conjugated to nerve agents surrogates. Toxicology, 411:15-23, 2019 Cited by PubMed Abstract: The efficiency of human butyrylcholinesterase (BChE) as a stoichiometric bioscavenger of nerve agents is well established. However, wide use is currently limited by production and purification costs. Aiming at identifying an alternative human protein bioscavenger, we looked for an original scaffold candidate by virtual screening of the Protein Data Bank for functional similarity using the "Surfing the Molecules" software (sumo-pbil.ibcp.fr) and a search model based on the BChE active site topology. Besides the expected acetylcholinesterase and butyrylcholinesterase, we identified a set of bile salt activated lipases structures, among which the human pancreatic lipase (hBAL) that shares 34% identity with BChE. We produced the recombinant enzyme in mammalian cells, purified it, and measured the inhibition constants for paraoxon and surrogates of VX, sarin and tabun. We solved the X-ray structure of apo hBAL and conjugates with paraoxon and the surrogates at resolutions in the 2-Å range. These structures allow the assessment of hBAL for scavenging nerve agents. They revealed that hBAL has inverted stereoselectivity for the surrogates of nerve agent compared to human cholinesterases. We observed a remarkable flip of the catalytic histidine driven by the chelation of Zn. Dealkylation of the conjugate, aka aging, was solely observed for paraoxon. PubMed: 30359675DOI: 10.1016/j.tox.2018.10.015 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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