6H02

Crystal structure of human Mediator subunit MED23

6H02 の概要

• エントリーDOI: 10.2210/pdb6h02/pdb
• 分子名称: Mediator of RNA polymerase II transcription subunit 23, LAMA nanobody NB106 (2 entities in total)
• 機能のキーワード: human, mediator, complex, subunit, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 173048.31

構造登録者

Clantin, B.,Monte, D.,Villeret, V. (登録日: 2018-07-06, 公開日: 2018-08-29, 最終更新日: 2024-10-23)

主引用文献

Monte, D.,Clantin, B.,Dewitte, F.,Lens, Z.,Rucktooa, P.,Pardon, E.,Steyaert, J.,Verger, A.,Villeret, V.
Crystal structure of human Mediator subunit MED23.
Nat Commun, 9:3389-3389, 2018

PubMed Abstract: The Mediator complex transduces regulatory information from enhancers to promoters and performs essential roles in the initiation of transcription in eukaryotes. Human Mediator comprises 26 subunits forming three modules termed Head, Middle and Tail. Here we present the 2.8 Å crystal structure of MED23, the largest subunit from the human Tail module. The structure identifies 25 HEAT repeats-like motifs organized into 5 α-solenoids. MED23 adopts an arch-shaped conformation, with an N-terminal domain (Nter) protruding from a large core region. In the core four solenoids, motifs wrap on themselves, creating triangular-shaped structural motifs on both faces of the arch, with extended grooves propagating through the interfaces between the solenoid motifs. MED23 is known to interact with several specific transcription activators and is involved in splicing, elongation, and post-transcriptional events. The structure rationalizes previous biochemical observations and paves the way for improved understanding of the cross-talk between Mediator and transcriptional activators.

PubMed: 30140054
DOI: 10.1038/s41467-018-05967-y

実験手法

X-RAY DIFFRACTION (2.8 Å)