6GZA

Structure of murine leukemia virus capsid C-terminal domain

6GZA の概要

• エントリーDOI: 10.2210/pdb6gza/pdb
• 分子名称: Capsid protein, COBALT (II) ION (3 entities in total)
• 機能のキーワード: capsid, dimer, cobalt, viral protein
• 由来する生物種: Murine leukemia virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19883.18

構造登録者

Qu, K.,Dolezal, M.,Schur, F.K.M.,Murciano, B.,Rumlova, M.,Ruml, T.,Briggs, J.A.G. (登録日: 2018-07-03, 公開日: 2018-12-05, 最終更新日: 2024-05-15)

主引用文献

Qu, K.,Glass, B.,Dolezal, M.,Schur, F.K.M.,Murciano, B.,Rein, A.,Rumlova, M.,Ruml, T.,Krausslich, H.G.,Briggs, J.A.G.
Structure and architecture of immature and mature murine leukemia virus capsids.
Proc. Natl. Acad. Sci. U.S.A., 115:E11751-E11760, 2018

PubMed Abstract: Retroviruses assemble and bud from infected cells in an immature form and require proteolytic maturation for infectivity. The CA (capsid) domains of the Gag polyproteins assemble a protein lattice as a truncated sphere in the immature virion. Proteolytic cleavage of Gag induces dramatic structural rearrangements; a subset of cleaved CA subsequently assembles into the mature core, whose architecture varies among retroviruses. Murine leukemia virus (MLV) is the prototypical γ-retrovirus and serves as the basis of retroviral vectors, but the structure of the MLV CA layer is unknown. Here we have combined X-ray crystallography with cryoelectron tomography to determine the structures of immature and mature MLV CA layers within authentic viral particles. This reveals the structural changes associated with maturation, and, by comparison with HIV-1, uncovers conserved and variable features. In contrast to HIV-1, most MLV CA is used for assembly of the mature core, which adopts variable, multilayered morphologies and does not form a closed structure. Unlike in HIV-1, there is similarity between protein-protein interfaces in the immature MLV CA layer and those in the mature CA layer, and structural maturation of MLV could be achieved through domain rotations that largely maintain hexameric interactions. Nevertheless, the dramatic architectural change on maturation indicates that extensive disassembly and reassembly are required for mature core growth. The core morphology suggests that wrapping of the genome in CA sheets may be sufficient to protect the MLV ribonucleoprotein during cell entry.

PubMed: 30478053
DOI: 10.1073/pnas.1811580115

実験手法

X-RAY DIFFRACTION (1.89 Å)