6GYV

Lariat-capping ribozyme (circular permutation form)

「4P95」から置き換えられました

6GYV の概要

• エントリーDOI: 10.2210/pdb6gyv/pdb
• 関連するPDBエントリー: 4P8Z 4P9R
• 分子名称: Lariat-capping ribozyme, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: lariat capping ribozyme, rna
• 由来する生物種: Didymium iridis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62158.54

構造登録者

Masquida, B.,Meyer, M.,Nielsen, H.,Olieric, V.,Roblin, P.,Johansen, S.D.,Westhof, E. (登録日: 2018-07-02, 公開日: 2018-08-22, 最終更新日: 2024-01-31)

主引用文献

Meyer, M.,Nielsen, H.,Olieric, V.,Roblin, P.,Johansen, S.D.,Westhof, E.,Masquida, B.
Speciation of a group I intron into a lariat capping ribozyme.
Proc. Natl. Acad. Sci. U.S.A., 111:7659-7664, 2014

PubMed Abstract: The lariat-capping (LC) ribozyme is a natural ribozyme isolated from eukaryotic microorganisms. Despite apparent structural similarity to group I introns, the LC ribozyme catalyzes cleavage by a 2',5' branching reaction, leaving the 3' product with a 3-nt lariat cap that functionally substitutes for a conventional mRNA cap in the downstream pre-mRNA encoding a homing endonuclease. We describe the crystal structures of the precleavage and postcleavage LC ribozymes, which suggest that structural features inherited from group I ribozymes have undergone speciation due to profound changes in molecular selection pressure, ultimately giving rise to an original branching ribozyme family. The structures elucidate the role of key elements that regulate the activity of the LC ribozyme by conformational switching and suggest a mechanism by which the signal for branching is transmitted to the catalytic core. The structures also show how conserved interactions twist residues, forming the lariat to join chemical groups involved in branching.

PubMed: 24821772
DOI: 10.1073/pnas.1322248111

実験手法

X-RAY DIFFRACTION (2.50003617357 Å)