6GYB

Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri

Summary for 6GYB

• Entry DOI: 10.2210/pdb6gyb/pdb
• EMDB information: 0089
• Descriptor: VirB7, VirB9 protein, VirB10 protein (3 entities in total)
• Functional Keywords: core complex, bacterial killing, protein transport, bacterial type iv secretion system, membrane protein
• Biological source: Xanthomonas axonopodis pv. citri (strain 306); More
• Total number of polymer chains: 42
• Total formula weight: 1225205.09

Authors

Sgro, G.G.,Costa, T.R.D.,Farah, C.S.,Waksman, G. (deposition date: 2018-06-28, release date: 2018-10-24, Last modification date: 2024-11-06)

Primary citation

Sgro, G.G.,Costa, T.R.D.,Cenens, W.,Souza, D.P.,Cassago, A.,Coutinho de Oliveira, L.,Salinas, R.K.,Portugal, R.V.,Farah, C.S.,Waksman, G.
Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri.
Nat Microbiol, 3:1429-1440, 2018

PubMed Abstract: Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 Å-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein-protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution.

PubMed: 30349081
DOI: 10.1038/s41564-018-0262-z

Experimental method

ELECTRON MICROSCOPY (3.28 Å)